Master Plant Sciences: First Year complete and detailed program
* UE 1A: Prokaryotic genetics 5 ects
=> THIS UNIT COULD BE CHANGED IN ITS PROGRAMME in 2016-2017: The programme is being determined:
Probably, other chapters will be developped in:
* MICROBIOLOGY (Chlamydomonas, Yeast, micro-algae...), Genetic regulation, Evolution
* CELL BIOLOGY of Eukaryotic organisms
* PLANT PHYSIOLOGY (Ecophysiology, Climate changes, Abiotic stresses, Biotic stresses....)
Previous progamme of this theoretical unit was:
- Chromosome Superstructure.
- Regulations of these phases: initiation of replication, transcription, and translation.
- The lactose operon paradigm and its generalization.
- Bacterial regulation Strategies: Two-component systems, cascade of the sigma factors antitermination of transcription, transcriptional attenuation translational attenuation quorum detectors. SOS system and DNA repair.
- Regulation of competence and transformation in Bacillus subtilis. -
- The bistability in regulation.
Lectures: 40h, Tutorial works: 10h
* UE 1B: Molecular eukaryotic genetics 5 ects
The theoretical unit contains two sub-parts: one concerns all eukaryotes, the other is more specific to plants.
Eukaryotic part:Base of gene expression in eukaryotes: Regulations molecular replication, repair, transcription and translation.
Plant Part: major gene regulation mechanisms in plants.- transcription factors in higher plants: general and example of the regulation of floral determinism - epigenetic regulation in plants
Lectures: 34h, Tutorial works: 12h
* UE 1C: Molecular and Biochemical Technologies 5 ects
Acquire knowledge and mastery of the current lab techniques in biochemistry, structural and molecular biology.
- General principles of chromatography, electrophoresis, immunorévélation, ELISA, capillary electrophoresis, HPLC, GC, FACE ..
- Planning of the practical work
Practical work: capillary electrophoresis, HPLC, GC, GC / MS, molecular modeling, two-dimensional electrophoresis, protein purification, molecular biology, FACE, Mudpit. ELISA, ...
Tutorial woks: 20h, Practical Lab: 30h
* UE 1D: Advanced Technologies in Analyses 5 ects
This second unit aims to deepen the knowledge base acquired in the first unit, and establish suitable strategies of extraction, identification, and analysis of biomolecules.
Most biological questions and models will be taken in the plant reign.
Tutorial woks: 20h, Practical Lab: 30h
* UE 1E: Current and Scientific English 5 ects
Situations favoring oral expression will be proposed by playing role games, oral presentations on scientfic topics, telephone interview simulation, technical information retrieval simulation from teachers or lecturers.
Tutorial works: 48h
* UE 1F: Practical Lab Project 5 ects
First, the different techniques used during the week will be presented. The student should understand their fundamental and technical bases (see also UE1C, UE1D), the advantages and limits, the suitable use in a biological strategy.
Secondly, the biological question will be proposed. Student will have to answer this question through the use of current techniques, selected and argued. Experiments will be performed on 8 half days. They will have to write a lab notebook (as learnt in UE1C and UE1D) and a short memory base on a scientific publication format.
Tutorial works: 10h, Practical Lab: 40h
Master Plant Sciences: Second Year complete and detailed program
* UE2-A : Lectures, Workshops and Seminars 20 ects
A– Plant Biopolymers : synthesis and biological functions (workshop coordinator: S Hawkins/C D’Hulst):
The structure, evolution and biosynthesis of major plant biopolymers (starch, cellulose, lignin and other higher plant cell wall polymers, as well as polymers from algae will be presented and their biological functions in planta will be discussed. Key research questions and experimental approaches (genomics, transcriptomics, proteomics, functional genomics, mutants…) will also be developed.
Lectures : 8h, Tutorial works : 6h, Workshop & Seminars : 12h
B – Plant Biopolymers : characterization and industrial utilizations (workshop coordinator: C Tokarski).
Different techniques for analyzing and characterizing plant biopolymers will be presented. Different industrial uses of plant polymers will be discussed (bio-plastics, bio-ethanol, composite materials, textiles…) and technological aspects (starch grain size, fiber length, cellulose crystallinity, lignin content etc.) impacting on polymer quality will be discussed.
Round Tables : 10hTD , Workshop & Seminars : 12h
C – Terrestrialization : From microbe to plants (workshop coordinator: S Ball).
Microbial adaptations and evolution to past climatic changes, the different origins of plant endosymbiosis (Streptophyta evolution), key steps during the terrestrialization of plants, introduction to plant- pathogen interactions.
Lectures: 8h, Tutorial works: 6h, Workshop & Seminars : 12h
D – Stress Adaptation Physiology (workshop coordinator: E Goulas).
The impact of abiotic stress (cold, drought, nitrogen, ozone) resulting from climate change, pollution and different industrial practices on plant and microbial biodiversity, growth and productivity will be discussed. Physiological responses and impacts on starch production, cell wall biosynthesis and secondary metabolite production will be discussed in the context of functional adaptation mechanisms and modifications of product quality.
Lectures: 8h, Tutorial works : 6h, Workshop & Seminars : 12h
Before workshop: the speakers sent publications to students for analysing before the seminars. He/she could also propose a subject for the bibliographic dissertation.
Courses: Some upgrade knowledge will be given
Workshop and Seminars (6h/day): two workshops is organised for each topic (8 workshops)
- Morning (3h): Seminar 1: to upgrade knowledge considering the heterogenous public origins, Seminar 2: on an original research projects. Each student is evaluated according to his/her questions during debate.
- Afternoon (3h): Roundtable: each student is evaluated according to his/her ilvolvement during the debate. Poster presentation: two students present poster to the speaker in order to be evaluated (public presentation).
Bibliographic dissertation: each student chooses one subject among those proposed by all speakers.
Assessment of participation in a scientific roundtable (topic B): 15%
Assessments of two oral presentations: 40%
Assessments of oral participation (questions): 15%
Assessment of bibliographic dissertation: 30%
* UE2-B: Adaptation genomics in plants 5 ects
This course aims at introducing the concepts and methodological approaches (e.g. top-down: QTLs and GWA-mapping; and bottom-up: genome scans) used to identify genomic regions and genes involved in major plant species adaptation. These concepts and approaches will be developed in the context of investigations on the genetic systems controlling plant breeding systems. They will also be illustrated by seminars presenting applications to other types of adaptations such as plant domestication, plant invasiveness, and resistance to herbivores. Finally, the student will also learn to manipulate genomic and transcriptomic data using elementary bioinformatic tools.
Lectures: 24h, Seminars: 12h, Practical work in Bioinformatics: 9h
The students will have to analyze a published paper in plant adaptation genomics in order to describe the methodological approach used, its theoretical grounds, and discuss the robustness of the results. They will also have to perform a mini-project involving analysis of data using bioinformatics tools.
Assessment of oral participation (paper analysis): 40%
Assessment of oral participation during conferences (question, debate): 20%
Assessment of mini project on analyses of pratical results in bioinformatics: 40%
* UE2-C : Employement, Ethics 5 ects
(Tutorial works: 48h)
EC1: Law, Ethics and Intellectual Property:
From original results presented at conferences (Workshops) of UE2-A, and subjects bibliographic submissions provided by lecturers, students together by pairs identify "results" they choose to value via this UE2-C.
Students will identify what they need (what supplements? Which people resources?) To assist in the transfer of laboratory results is through an assignment or license of a patent or through the creation of a start up. To assist in this, students will have a series of "theoretical" lectures on the value chain of scientific results. Four themes will be addressed: Concept and definition of the chain of research development, Concept and definition of intellectual property (invention disclosure, patent law software, database protection, copyrights ..) Concept and definition of a contract, Notion and legal definitions (of a patent license, ownership, licensing, license assignment, legal negotiations, NES, MTA, service contract ...),. Through these themes, students will be sensitized to the different professions related to the development and transfer of assignments: Thematic Expert (Research and Development Engineer), Business Developer (charge d'affaires), patent engineer, lawyer ... ..
These lectures will be supplemented by other healthy particular:
• Support the creation of innovative business and technological (payroll, mass loaded, recruitment ...) via professionals from an incubator (CRE INNOV, HubInnovation, Haute Borne, Villeneuve d'Ascq)
• GMO, varietal creations, patents on processes and methods for obtaining plants, participate in the drafting of a specific folder manipulation of transgenic organisms (eg a greenhouse S2 file)
• Awareness mounting a public research project (type ANR), properties of the results of scientific publications
EC2 :professionnal employment (cover letter, CV, telephone).